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class="meau"> <span id="iwlmo9c" class="m1"></span> <span id="iwlmo9c" class="m2"></span> <span id="iwlmo9c" class="m3"></span> </div> <div id="iwlmo9c" class="Logo fl"><h1></h1></div> <div id="iwlmo9c" class="toptel"></div> <div id="iwlmo9c" class="clear"></div> </div> <div id="iwlmo9c" class="Nav w100"> <div id="iwlmo9c" class="Navlist w1000"> <ul> <li id="Top1"></li> </ul> <div id="iwlmo9c" class="clear"></div> </div> <div id="iwlmo9c" class="clear"></div> </div> <div id="iwlmo9c" class="clear"></div> </div> <div id="iwlmo9c" class="blockindex h450"> <div id="iwlmo9c" class="new9"> <div id="iwlmo9c" class="new91"> <h1>鑒別ELISA試劑盒真?zhèn)蔚姆椒?/h1> 發(fā)布時(shí)間：2024-02-27 </div> <div id="iwlmo9c" class="new92"> <div class="iwlmo9c" id="contentt"></div> 鑒別elisa試劑盒真?zhèn)蔚姆椒ǎ?br>一．利用干擾實(shí)驗(yàn)即可辨別elisa試劑盒是否檢測目的抗原，方法如下：<br>(1) 將針對試劑盒特異性的重組蛋白抗原和試劑盒中的檢測抗體配置成antibody: antigen≈1:2的混合孵育液<br>(2) 37℃孵育15分鐘后，代替原試劑盒中的檢測抗體<br>(3) 后續(xù)操作按照試劑盒說明書進(jìn)行，加檢測抗體、酶復(fù)合物和底物<br>(4) 實(shí)驗(yàn)完畢后，檢測其標(biāo)準(zhǔn)曲線，如果標(biāo)準(zhǔn)曲線良好則說明加入的目的抗原和試劑盒抗體不匹配，試劑盒檢測抗體針對的是非目的抗原，該試劑盒為偽劣假造elisa試劑盒。<br>(5) 如果標(biāo)準(zhǔn)曲線無線性，則說明試劑盒檢測抗體已被目的抗原中和或干擾，影響了其實(shí)際試劑盒抗體的檢測作用，則該試劑盒檢測抗體針對的是目的抗原。<br>二．如果購買了兩盒同一公司的elisa試劑盒a和b，利用交叉實(shí)驗(yàn)即可辨別elisa試劑盒是否造假，方法如下：<br>(1) 取出購買的試劑盒a的包被板兩條。<br>(2) 一條包被板加試劑盒a的標(biāo)準(zhǔn)品做標(biāo)準(zhǔn)曲線。<br>(3) 另一條包被板加試劑盒b的標(biāo)準(zhǔn)品做標(biāo)準(zhǔn)曲線。<br>(4) 后續(xù)操作按照試劑盒說明書進(jìn)行，加檢測抗體、酶復(fù)合物和底物。<br>(5) 實(shí)驗(yàn)完畢后，檢測兩條標(biāo)準(zhǔn)曲線，如果兩條<br>標(biāo)準(zhǔn)曲線一致則說明兩個(gè)elisa試劑盒檢測的同一個(gè)指標(biāo)而非a和b，即該試劑盒為偽劣假造elisa試劑盒。<br>(6) 如果兩條標(biāo)準(zhǔn)曲線不一致則說明兩個(gè)elisa試劑盒檢測的不同指標(biāo)，試劑盒a只能檢測a，不能檢測b，即該試劑盒為正常的elisa試劑盒。<br> <div class="iwlmo9c" id="contentb"></div> </div> <div id="iwlmo9c" class="clearfix"></div> </div> <div>上一個(gè)：<a href="n355463.htm">RTT061432FTP現(xiàn)貨庫存，最新價(jià)格</a><br /> 下一個(gè)：<a href="n355461.htm">組裝臺式機(jī)二手估價(jià)，求二手臺式組裝電腦的價(jià)格</a> <br /><br /> <a href="n355163.htm">win8畫圖在哪里(win8.1圖形設(shè)置在哪里)</a><br 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